Effect of Cooking Methods on Vitamin C in Potatoes


PURPOSE

The purpose of this experiment was to determine the effect of different cooking methods on the vitamin C content of potatoes.

I became interested in this idea when I discovered that vitamin C is very important to human health, and also that exposure to various things decreases or hurts the vitamin C content.  Light, oxygen, metal (especially iron), heat, and some chemicals all reduce the vitamin C content.  

The information gained from this experiment should benefit health conscious people who are seeking to be healthier, by letting them know which cooking method minimizes the vitamin C loss in potatoes or other vegetables.  Also it would basically help all of society maintain better health.    
Cooking Methods on Vitamin C in Potatoes

HYPOTHESIS

My first hypothesis was that the longer time potatoes are steamed, the lower the vitamin C content would be.

My second hypothesis was that boiling potatoes in water would be more damaging to vitamin C than steaming would be.

I base my second hypothesis on the definition of vitamin C in Merriam-Webster’s Medical Desk Dictionary, “Vitamin C is a water-soluble vitamin.”
So therefore, if a potato is sitting in the water for a long period of time, it will lose some of the vitamin C content in it.

                                         EXPERIMENT DESIGN

The constants in this study were:
  •   The type and size of the chunks of the potato
  •   The temperature potatoes are cooked at (212 degrees F.)
  •   The time potatoes are cooked (15, 30, and 45 minutes)
  •   Amount of metal each potato is exposed to
  •   Amount of water potatoes are cooked in during boiling test
  •   Method for measuring vitamin C

The manipulated variable was the cooking method. 

The responding variable was the amount of vitamin C in the potatoes.

To measure the responding variable I determined the vitamin C content using an iodine, weak sulfuric acid method at a local industrial food-processing laboratory.

MATERIALS
   

QUANTITY

ITEMS DESCRIPTION
18
Potatoes
1
 Pot
1
Timer
1

Measuring Cup (250 milliliters)
6
Liters of water
7
Vacuum pack bags
1
Vacuum packer
1
Magnetic Mixer
1
  Flask
1
Hand-held Mixer
1
Durette
1
  Repipettor


PROCEDURES

1. Prepare potatoes for cooking

a. Chop potatoes into 2.5 centimeter cubes

b. Thoroughly but briefly rinse the chopped potatoes in cold water

2.  Boil potatoes

a. Put 1000 milliliters of water into a pot

b. Add 750 milliliters of potatoes

c. Set temperature so pot boils continuously 

d. Start timer at first boil

e. Take 250 milliliters of potatoes out of the boiling water when the timer goes off at 15 minutes, continue cooking remainder

f. Take 250 milliliters of potatoes out when timer goes off at 30 minutes, continue cooking remainder

g. Take 250 milliliters of potatoes out at 45 minutes.

3.  Package and store samples

a. Let each batch of boiled potatoes cool in colander for 10 minutes 

b. Vacuum pack potatoes so they aren’t touching any air

c. Label each vacuum pack according to the cooking method and time

d. Set the packs with the potatoes in them into the refrigerator 

4.  Steaming potatoes

a. Put 250 milliliters of water in a pot

b. Set a steaming basket above the water in the pot

c. Repeat steps 2.d – 2.g except while steaming potatoes

d. Repeat step 3 for the steamed groups   

5.  Measure vitamin C

a. Collect a sample potatoes

b. Place 600 milliliter beaker onto the electronic balance and tare, add 100.0 grams of sample

c. Add 300 milliliters of distilled or deionized water

d. Add 5 milliliters 10% H2SO4 (sulfuric acid) using a repipettor 

e. Add 5 milliliters 1% starch solution to mixture

f. Titrate mixture with iodine solution to a blue-black end point that persists for not less that 20 seconds

g. Record the amount of 0.10 N iodine solution used 

6. Formula for calculating vitamin C content in potatoes

a. Take the squared milliliter of iodine and subtract 0.5 from it

b. Multiply that answer by 0.1

c. Multiply that answer by 88

d. Divide that answer by the weight of the potatoes


RESULTS

The original purpose of this experiment was to see which cooking method, steaming vs. boiling, damaged the vitamin C in a potato the most.

The results of the experiment were that overall boiling was more damaging to the vitamin C in a potato than steaming was.  When I steamed the potatoes for 15 minutes, there was 7.2 milligrams of vitamin C left in the potato, after 30 minutes they had 8.4 milligrams of vitamin C left in the potato, 45 minutes had 7.0 milligrams of vitamin C left.

The potatoes that were boiled for 15 minutes had 3.9 milligrams of vitamin C, after 30 minutes they had 7.0 milligrams of vitamin C left, 45 minutes had 5.6 milligrams of vitamin C left in the potato.

CONCLUSION

My first hypothesis was that boiling the potatoes would be more damaging to the vitamin C in potatoes than steaming would be.

The results indicate that my first hypothesis should be accepted, because boiled potatoes had less vitamin C than the steamed did.

My second hypothesis was that the longer the potatoes were cooked the more damaging it would be to the vitamin C in the potatoes.

The results indicated that my second hypothesis should be rejected.  My data suggests that potatoes cooked 30 minutes had more vitamin C than those cooked either 15 or 45 minutes.  This is puzzling.  Maybe the potatoes have to cook until soft, so the vitamin C is released from the cells.

Because of the results of this experiment, I wonder if any other cooking method, like pressure-cooking or deep-frying would be more or less damaging to the vitamin C in potatoes. I also wonder if the results would be affected if a different type of potato were tested.

If I were to conduct this project again I would definitely have more trial runs.  During my experiment I accidentally burned one batch of my potatoes, and I’m not sure if that affected the results or not.  I should probably have cooked a new batch to replace the burnt batch.  I should also have added a 60 minute cooking time group.



Researched by ---- Colby D.



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How Different Soils Affect The Growth of a Pinto Bean

                                                    

 PURPOSE

The purpose of this experiment was to see how different soils affect the growth of a pinto bean. 

I became interested in this idea when Don Jordan, my grandfather, was describing different types of soils and how they affect the growth of a plant. 

The information gained from this experiment will help produce more trees and plants faster, so they can clean the polluted air around the plant's area. Also knowing this information will help farmers produce more vegetable 


                                                  HYPOTHESIS

My hypothesis is that the cotton would increase the growth of a pinto bean the most. 

I base my hypothesis on Don Jordan, my grandfather, who did this experiment before me and discovered that cotton was the best material to grow pinto beans. 

Experimental Design

The constants in this study were:
  • The amount of water and light
  • Same type of plant
  • Same amount of bedding material
  • Same seed depth
  • Same planting cups
  • Same temperature
The responding variable was how tall my plant grows. 

To measure my responding variable I will use a metric ruler. Growth will be checked weekly. 

MATERIALS


Quantity
Item Description
12
Western Family foam cups 473ml
12
Western Family pinto beans 100% 907.2g
240 ml
Nursery mix (potting soil) (composted forest bark, pine needles, leaves, and fertilizer)
240 ml
Moxee dirt (regular soil from Long Sing Hop Yard)
240 ml
Blended soil (mix of 30 year aged bark mulched, top soil and sand)
24
Cotton balls (100%cotton) jumbo size
1
"Sharpie" permanent marker
3
240 ml measuring cups

Tap water
  

PROCEDURES

Label 3 cups with the words "cotton"

Label 3 cups with the words "nursery"

Label 3 cups with the words "blended"

Label 3 cups with the words "moxee"

Take a measuring cup and measure 240 ml of Nursery Mix.

Fill one cup with the words "nursery" on them with this soil.

Repeat this with the other two cups with the words "nursery" on them.

Repeat steps 5 - 7 with the cups labeled "Blended" and "Moxee", but use the appropriate soil for each labeled cup.

Spread 4 cotton balls at the bottom of each of the 3 cups marked "cotton".

Take your pinky finger and push it down into the dirt about 2 cm.

Do this in all the cups labeled "nursery", "blended", and "moxee".

Put one pinto bean in every hole. Make sure that the curve in the bean is faced upward.

Next cover the beans that are in the soils with the soil that are in the cup, do this to all the beans that are in the cups full of soil.

Put one bean on top of the cotton in the cups labeled cotton.

For each cup labeled cotton spread 4 more cotton balls over each bean.

Take 24 ml of water with the syringe and squirt the water into one cup.

Do step 16 for each seed that you planted.

Repeat steps 16 - 17 every other day for 6 weeks.

Set all plants side-by-side under 1 light source.

Rotate plants 90 degrees to the right every day.

Measure each plant at the end of each week for 6 weeks.

RESULTS

The original purpose of this experiment was to see how different materials affect the growth of a pinto bean. 

The results of this experiment were that a pinto bean grows best in cotton. 

Conclusions

My hypothesis was that the cotton would be the best material for the pinto bean to grow in. 

The results indicate that this hypothesis should be accepted. The pinto bean grew best in the cotton. 

If I were to do this experiment again I would start with many more cups of each type, so that more would grow. I would also use some soils that were not as powdery as the Moxee or the Blended soils. They compacted too much and kept the plants from growing. I would also repeat my experiment another time or two from the very beginning to make sure my results were reliable. 

Because of my results I wonder how wheat or corn would compare to beans if grown in different soils. 

Researched by --- Morgan M



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Effect of Metal Type on the Voltage of a Galvanic Cell


PURPOSE

The purpose of this experiment was to determine the effect of electrode combinations on the voltage of a galvanic cell.

I became interested in this idea when I was listening to my CD player and wondered how the battery made energy for the CD player to use.

The information gained from this experiment would help all wet cell battery users by finding metals with more electrical output. Almost everyone in our society uses these batteries.


Voltage of a Galvanic Cell



HYPOTHESIS

My hypothesis was that a zinc anode and copper cathode would create the most voltage output.

I based my hypothesis on an article in the Hutchison Dictionary of Science that stated, “The first galvanic battery that was made by Luigi Galvani used zinc as a anode and copper as a cathode which still proves to be efficient.” 


 
EXPERIMENT DESIGN

The constants in this study were:

  • The size of the metal electrod
  • Amount of electrolyte
  • Size of beaker 
  • Voltmeter
  • Temperature

The manipulated variable was the combinations I use as electrodes to create a galvanic cell.

The responding variable was the electrical output. 

To measure the responding variable I used a voltmeter to measure the galvanic cell’s electrical potential. 

MATERIALS

QUANTITY
ITEM DESCRIPTION
 1 
Voltmeter
1
Glass Beaker
2
Zinc    2cm x 12cm
2
Copper   2cm x 12cm
2
Lead    2cm x 12cm
200ml
Copper Nitrate
200ml
Zinc Nitrate
200ml
Lead Nitrate
3
Porous cups


PROCEDURES

1. Clean the copper, lead and zinc strips before starting the experiment. Sand with a fine grade sand paper to take off the outside coating.

2. Place 50ml of the matching electrolyte with the metal strip you’re using in the beaker.

3. Take another electrolyte and add 50ml to the porous and add the matching metal.

4. Attach the voltmeter’s ends to the two metals.

5. Put the porous cup in the beaker.

6. Immediately take volt recordings in mini volts.

7. Repeat steps 2-5 3 times.

8. After conducting all of the experiment clean up all of the mess from conducting the experiment. 

RESULTS

The original purpose of this experiment was to determine the effect of electrode combinations on the voltage of a galvanic cell.

The results of the experiment were the lead-copper test created the most potential at 481.2 mv. The lead-zinc test created 106.4 mv. The zinc-copper test created 82.3 mv.


CONCLUSION

My hypothesis was that a zinc anode and copper cathode would create the most voltage output.

The results indicate that this hypothesis should be rejected for the lead-copper galvanic cell created more energy.

Because of the results of this experiment, I wonder if using sulfates or carbonates instead of nitrates as electrolytes would change the electrical output. Another thing that could be changed is the temperature. I did this experiment at room temperature (20∞C). You could do it at 0∞C  (freezing) or at about 40∞C, which is like a hot summer day.   

If I were to conduct this project again I would try to use more anode cathode combinations. I would conduct more trials. I would use a voltmeter that was very precise and accurate below one volt. I would repeat the entire experiment, using fresh electrolytes and electrodes. I would also soak the porous cups for a few hours before the experiment.



Researched by Derek L.


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Dissolution Rate Of Ibuprofen Pills

PURPOSE

The purpose of this experiment was to determine the rate of dissolution for different brands of Ibuprofen. 

I became interested in this idea when I had a headache and took an Advil and I decided that it would be a good idea to see which brand would cure my headache the fastest.

The information gained from this experiment could benefit every person that has had a headache and didn’t get the fastest relief possible. I think that everyone has the need to get his or her headaches relieved as soon as possible.


Dissolution Rate Of Ibuprofen Pills

HYPOTHESIS

My hypothesis was that the roughest and smallest pill would dissolve the quickest.

I based my hypothesis on the fact that the smallest and roughest pill would break up the quickest because it isn’t as well compacted as the others.


EXPERIMENT DESIGN


The constants in this study were:
  •  Amount of water (250ml) for each test
  •  Temperature of water (37∞ Celsius)
  •  Speed of magnetic stirrer (high setting)
  •  Type of water (tap)
  •  Amount of pills (one) tested at one time
  •  Testing procedures
  •  Type of stopwatch 
  •  Type of pill (Ibuprofen)
The manipulated variable was the brand of Ibuprofen.

The responding variable was the dissolution time. 

To measure the responding variable I used a stopwatch accurate to 0.01.


MATERIALS
   
                   
QUANTITY
ITEM DESCRIPTION
5
Ibuprofen for each of the 4 brands
2                  
magnetic stirrers
600ml
tap water
2  
300 ml glass beakers
1   
Celsius thermometer
   pair of heat resistant gloves
1
thick lab apron
1 set 
goggles
1
  microwave
             


PROCEDURES

1. Measure 250ml of tap water

2. Pour 250ml of water into a microwave safe container 

3. Heat water in microwave to 37∞ Celsius
4. Pour heated water into 300 ml glass beaker (Only up to the 250 mark though)

5. Put onto magnetic stirrer’s base

6. Drop in magnetic stirring capsule 

7. Turn on magnetic stirrer to high (Let the stirrer get going fast before you drop in the pill)

8. Drop in first brand of Ibuprofen pill

9. Immediately Start stopwatch

10. When the pill dissolves (disappears) stop the stop watch then the stirrer

11. Record the dissolution time of the pill in minutes and seconds

12. Repeat steps 1-11 for the other pills of this same brand (5 more times)

13. Repeat steps 1-12 using the next brand of pills

14. Repeat steps 1-13 with the remaining brands 

15. Average the dissolution rate for each brand.

16. Compare brands


RESULTS

The original purpose of this experiment was to determine the Dissolution rate of different brands of Ibuprofen.

The results of the experiment were that the brand Advil had the fastest dissolution rate.

CONCLUSION

My hypothesis was that the smallest and smoothest pill would dissolve the quickest because it is the smallest.

The results indicate that this hypothesis should be accepted.

Because of the results of this experiment, I wonder if I compared the rate of dissolution of lactase pills and Advil pills and see which one would dissolve the quickest.

If I were to conduct this project again I would try seeing if different temperatures of water had any effect on the dissolution rate.



Researched by --- Baylee B



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